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- 文献和实验
- 技术资料
- 库存:
999
- 供应商:
北京泽平
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热销品大量现货,其余请咨询
- 保修期:
1年
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EA
货号:T_70107-764-704
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英文名称:CORNING PIPETTOR 20-200UL 1CS
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文献和实验Large-scale yeast transformation
transformations (90 mls cells). Grow 50 ml culture in 2xSC-leu 1 , overnight (light innoculum, ie 20 ul of cells). Next morning, add 40 mls YPAD, grow 2-3 hours more. Spin down 100 ul of the culture (=1 transformation's worth) and check
Large-scale yeast transformation大规模转化酵母【Yale University】
in 2xSC-leu 1 , overnight (light innoculum, ie 20 ul of cells). Next morning, add 40 mls YPAD, grow 2-3 hours more. Spin down 100 ul of the culture (=1 transformation's worth) and check pellet size. Should be approx 3 ul. If much greater, use
. and resuspend in 1.5mL TE/LiAC. Add 100ul cells to the plasmid DNAs (1-5 ug) and single-stranded carrier DNA (20ug) in a microfuge tube. The carrier DNA is produced by dissolving salmon sperm DNA in TE, sonicating it to reduce its viscosity, extracting
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