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货号:T_70312304119
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英文名称:X1000 Large spoon, 8ML PS white, L150, Sterile SAL 10-3, 10x100/Bags
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
overnight or -70°C for 15 min. 13. Spin 12000g, 10 min, 4°C. Wash pellet with 70 EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12.
A quick RNA mini-prep for Neurospora mycelial cultures
pellet with 70% EtOH and drain. Pellet should be a light pink or white. 14. Resuspend in 0.4 ml sterile gd H2 O in a microfuge tube. Precipitate with NaOAc and EtOH as in step 12. 15. Spin 10-20 min in microfuge. Wash twice with 70% EtOH and dry
, store frozen. Methanol: FISHER SCIENTIFIC cat# A412-500. Methyl alcohol anhydrous (ACS)(Absolute) Acetone free, 500ml bottle. Nutrient Mix F-10: GIBCO cat# 81200-040. Nutrient Mixture F-10 (HAM) powdered form, prepared to instructions, 10 x
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