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- T_701MP113037032
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_701MP113037032
中文名称：
英文名称：M9 MINIMAL MEDIUM SALTS 1KG
货期：热销品现货，其他请咨询
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文献和实验Expression Protocol in M9 Minimal Media via T7 Promoter
NaCl H2O to final 1L volume and autoclave To prepare 500mL M9 minimal media; 100mL of 5xM9 salts 1 mL 1M MgSO4 50 uL 1M CaCl2 5 mL 100x Basal Medium Eagle Vitamin Solution (Gibco) 2.5 mL filter sterilized NH
Expression Protocol in M9 Minimal Media via T7 Promoter
Expression Protocol in M9 Minimal Media via T7 Promoter The following protocol has been used successfully to 15 N or 13 C/15 N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins
for M13 viral infection (9), JM101 is streaked onto a M9 minimal media (modified from that given in reference (1) plate and XL1BMRF' is streaked onto an LB plate (1) containing tetracycline. ED8767 is streaked onto an LB plate. These plates are incubated
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