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货号：T_701ES5500
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英文名称：HEINZ BODY STAIN 100ML 2PK
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文献和实验Preparation and Immunostaining of Polytene Chromosome Squashes
and mounted in Vectashield (Vecta Laboratories) containing DAPI (1µg/ml) as a DNA counter-stain. The coverslip was sealed with nail varnish before viewing under the microscope. [1] [2] 下一页
. Remove coverslip. 13. Place slide on a paper towel and stain the nuclei/chromosomes by covering the slide with Hoechst 33342 [1 microliter Hoechst 33342 (stock: 0.1mg/ml in water) in 1 ml distilled water] for 5 minutes. Wear gloves when working
units/ml). Now, I don’t know what a ‘unit’ of phalloidin is, but this stock is effectively 100X. 2) For DAPI (D-1306 from Molecular Probes), we dilute the supplied 10mg in 9.5mls of H2O, giving a stock of 3mM. This is ~5x less concentrated
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