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- 2025年07月15日
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999
- 供应商：
北京泽平
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- 保修期：
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- 规格：
EA
货号：T_70109-841-002
中文名称：
英文名称：PRESSURE BTL CLR 500ML 10CS
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文献和实验A safe method of extracting DNA from Coccidioides immitis
in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers (Identi-plug, Fisher). When there has been sufficient growth
A safe method of extracting DNA from Coccidioides immitis
from any pathogenic fungus. Cultures of C. immitis are grown in the saprophytic phase at 37 C in 100 ml of 2X GYE medium (20 g glucose and 10 g yeast extract in 1 liter medium, autoclaved for 15 min.) in 500 ml plastic Nalgene flasks with plastic foam stoppers
6000-8000 200 mM potassium phosphate, pH 7.5 30 mM potassium citrate, pH 7.5 Mix 10 mL 1M K phosphate pH 7.5, 5 ml 300 mM K citrate, pH 7.5, 10 g PEG, and ~25 ml H20, stir ~10 min to dissolve the PEG, and add more H20 to final volume of 50
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