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文献和实验V294.PART II，Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
-mercaptoethanol (Sigma; cat. no. M7522) diluted in 10 mL of RPMIcontaining antibiotics; this can be stored at 4°C. The BMMs are differentiatedand maintained in macrophage growth medium, which consists of macrophagestarve medium with the addition of 15% conditioned
Fibroblast Cell Systems Instructions for Use
/EDTA Solution (T/E) (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (TNS) (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A. Product Applications Clonetics® Normal Human NHDF and NHLF
Endothelial Cell Systems - Instructions For Use
the flask. For each 25 cm2 of cells to be subcultured, allow 3 ml of Clonetics® Trypsin/EDTA (T/E) to thaw and come to room temperature. For each 25 cm2 of cells to be subcultured, allow 5 ml of Clonetics® HEPES Buffered Saline Solution (HEPES-BSS
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