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999
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北京泽平
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货号：T_70313214429
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英文名称：15M TUBE NON DEHP 8X14,4MM
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文献和实验.These are only rough guidelines. Detailed instructions about PCR laboratory setup and maintenance may be found in PCR Methods and Applications, 3, 2, S1-S14, 1993.Preparation of Reaction Mixture To perform several parallel reactions, we recommend the preparation
Differential Display of Cotton Transcripts
for 15 min. Add 1.5 ul M-MuLV RT, gently mix. Incubate at 37C for 1 hour. Heat the reaction in 95C water bath for 5 min, store cDNA at -20C for later use. PCR Amplification To a 0.5 ml microcentrifuge tube, add 4.0 ul cDNA
Differential Display of Cotton Transcripts
, Madison, WI) 20 uM decamer primers (random) 20 uM anchored oligo-dT12MN primers (M=A,C, or G; N=A,C,G, or T) mixed dNTPs (2.5 mM each dNTP, 100 uM each dNTP Taq DNA Polymerase and buffer (Promega, Madison, WI
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