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- 文献和实验
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999
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北京泽平
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货号:T_70312353820
中文名称:
英文名称:X750 GRIND MEDIA ZIRCONIA 10X10MM
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文献和实验), Nucleic Acid Electrophoresis (Springer Lab Manual). Heidelberg: Springer. pp 1–53. 2.Thermo Fisher Scientific Inc (2010) Nucleic Acid Detection and Analysis. In: Molecular Probes Handbook: A Guide to Fluorescent Probes and Labeling Technologies. pp 349–360
Protocols for protein extraction from whole tissues for isoelectric focusing
Physiology 81:802-806). 1.Grind 1 g of fresh tissue to a powder with liquid nitrogen in a mortar and pestle. 2.Add 2.5 ml of Tris pH8.8 buffered phenol and 2.5 ml of extraction media (0.1 M Tris-HCl pH 8.8,10 mM EDTA,0.4% 2-mercaptoethanol,0.9 M sucrose
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
O to 1 liter. Autoclave and store at room temperature.- 10x Taq Buffer: 500 mM KCl, 100 mM Tris-HCl (pH 9.0 at 25oC), 1% Triton X-100. - 20x SSC: 3 M NaCl, 0.3 M Na3Citrate-2H20. Store at room temperature.- Mounting media: 70% glycerol, 1mg/ml p
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