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- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：4651230N
中文名称：
英文名称：F1-ClipTip Fixed 100 µl
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文献和实验Immunohistochemistry on fixed, paraffin-embedded sections
. If one of your antibody is biotin-conjugated, you need at this point to do endogenous biotin blocking . 4- blot the slides without washing and apply the primary antibody (100µl), in a moist chamber, at RT for 1-18 hr. 5- Wash twice in TBS-T. 5 min / wash
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
(37%, Fisher, Cat# F79-500)- Formamide (Boehringer Mannheim Cat# 100 731)- Glycerol (Boehringer Mannheim Cat#100 649)- Glycine (Sigma Cat# G-4392)- Glycogen (Boeringher Mannheim Cat# 901-393)- Heparin (Sigma, Cat# H-3393)- Hepes
Double immunohistochemistry for BCL6 and PNA for Germinal Center detection
, at RT overnight . Rabbit anti BCL-6 (Santa Cruz Biotechnology, Santa Cruz, CA: Bcl-6 (N-3): sc-858, use at 0.5 µg/ml) 5- Wash twice in TBS-T. 6- add the biotin-conjugated secondary antibody (100 to 200 µl) and incubate for 45 min
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