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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 库存:
现货
- 供应商:
联硕
- CAS号:
9025-57-4
- 规格:
1瓶
应用
生化/生理作用

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文献和实验ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
ml of buffer A plus 0.5% Triton X-100 (Sigma) with a homogenizer (55 ml glass pestle unit). Centrifuge in two 30 ml Corex tubes at 8000 rpm for 10 min. in a Beckman JS-13 rotor. Repeat step 7, centrifuge at 6000 rpm for 10 min in a JS-13
Composition29 ml45 mlComponent5%7.5%10% 5%7.5%10%water16.113.611.7 24.1520.417.55Soln. B7.57.57.5 11.2511.2511.25SDS0.30.30.3 0.450.450.45Soln A57.510 7.511.2515TEMED (µl)151515 202020persulfate (µl)150150150 200200200 Reducing Buffer (1:1)Reducing Buffer (1/3 X
.6. Load 10μl- 25μl(10μl for a 15 tooth mini comb) and then overlay samples with 1 x SDS running buffer. Empty wells should have 10-15μl sample buffer added and be overlayed as with the test samples so the gel runs straight. Flood the upper chamber
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