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- Sino Biological
- 北京
- CV011
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
pCMV3-untagged Negative Control Vector
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
1 Unit
pCMV3-untagged-NCV (Negative Control Vector) Physical Map
Description
| Vector Name | pCMV3-untagged-NCV |
| Vector Size | 6025bp |
| Vector Type | Mammalian Expression Vector |
| Expression Method | Constitutive, Stable / Transient |
| Promoter | CMV |
| Antibiotic Resistance | Ampicillin |
| Selection In Mammalian Cells | Hygromycin |
| Protein Tag | None |
| Sequencing Primer | Forward:T7(TAATACGACTCACTATAGGG) Reverse:BGH(TAGAAGGCACAGTCGAGG) |
Schematic of pCMV3-untagged-NCV (Negative Control Vector) Multiple Cloning Sites
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文献和实验该产品被引用文献
1, Zhang H, et al. Epidermal growth factor promotes proliferation of dermal papilla cells via Notch signaling pathway.Biochimie, PubMed ID: 27109378
2, Yu W, et al. Plexin-B2 Mediates Physiologic and Pathologic Functions of Angiogenin.Cell, PubMed ID: 29100074
3, Rogers TJ, et al. Reversal of Triple-negative Breast Cancer EMT by miR-200c Decreases Tryptophan Catabolism and a Program of Immune-Suppression.Molecular cancer research : MCR, PubMed ID: 30213797
4, Sarina , et al. Mechanism of Placenta Damage in Gestational Diabetes Mellitus by Investigating TXNIP of Patient Samples and Gene Functional Research in Cell Line.Diabetes therapy : research, treatment and education of diabetes and related disorders, PubMed ID: 31654346
5, Kim D, et al. Muscle-derived TRAIL negatively regulates myogenic differentiation.Experimental cell research, PubMed ID: 32645396
6, Stucki D, et al. Endogenous Carbon Monoxide Signaling Modulates Mitochondrial Function and Intracellular Glucose Utilization: Impact of the Heme Oxygenase Substrate Hemin.Antioxidants (Basel, Switzerland), PubMed ID: 32717801
7, Chen R, et al. SNAIL regulates gastric carcinogenesis through CCN3 and NEFL.Carcinogenesis, PubMed ID: 33313663
8, Sharma A, et al. Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes.Microorganisms, PubMed ID: 34073047
9, Sun JH, et al. Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction.Neural regeneration research, PubMed ID: 34558529
10, Wei W, et al. The Role of Ferroptosis Signature in Overall Survival and Chemotherapy of Pancreatic Adenocarcinoma.DNA and cell biology, PubMed ID: 34898275
11, Hung YH, et al. Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.American journal of cancer research, PubMed ID: 35261797
12, Kuang Y, et al. NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation.Molecular medicine reports, PubMed ID: 35425997
13, Burnie J, et al. P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is incorporated into clinical HIV-1 isolates and can mediate virus capture and subsequent transfer to permissive cells.Retrovirology, PubMed ID: 35597982
14, Ma WL, et al. Ursolic acid silences CYP19A1/aromatase to suppress gastric cancer growth.Cancer medicine, PubMed ID: 35545835
15, Kim NY, et al. Euphorbiasteroid Abrogates EGFR and Wnt/β-Catenin Signaling in Non-Small-Cell Lung Cancer Cells to Impart Anticancer Activity.Molecules (Basel, Switzerland), PubMed ID: 35744950
16, Shonibare Z, et al. Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer.Cell reports, PubMed ID: 35905726
17, Adams LM, et al. Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling.The Journal of biological chemistry, PubMed ID: 36470426
18, Sakamoto A, et al. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel Mediates Acrolein Cytotoxicity in Human Lung Cancer Cells.International journal of molecular sciences, PubMed ID: 37511605
19, Maru B, et al. PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy.Cell reports. Medicine, PubMed ID: 37683650
20, Sun Y, et al. GNA15 facilitates the malignant development of thyroid carcinoma cells via the BTK-mediated MAPK signaling pathway.Histology and histopathology, PubMed ID: 38333922
21, Wang H, et al. The regulatory role of miR-21 in ferroptosis by targeting FTH1 and the contribution of microglia-derived miR-21 in exosomes to arsenic-induced neuronal ferroptosis.Journal of hazardous materials, PubMed ID: 39186845
2, Yu W, et al. Plexin-B2 Mediates Physiologic and Pathologic Functions of Angiogenin.Cell, PubMed ID: 29100074
3, Rogers TJ, et al. Reversal of Triple-negative Breast Cancer EMT by miR-200c Decreases Tryptophan Catabolism and a Program of Immune-Suppression.Molecular cancer research : MCR, PubMed ID: 30213797
4, Sarina , et al. Mechanism of Placenta Damage in Gestational Diabetes Mellitus by Investigating TXNIP of Patient Samples and Gene Functional Research in Cell Line.Diabetes therapy : research, treatment and education of diabetes and related disorders, PubMed ID: 31654346
5, Kim D, et al. Muscle-derived TRAIL negatively regulates myogenic differentiation.Experimental cell research, PubMed ID: 32645396
6, Stucki D, et al. Endogenous Carbon Monoxide Signaling Modulates Mitochondrial Function and Intracellular Glucose Utilization: Impact of the Heme Oxygenase Substrate Hemin.Antioxidants (Basel, Switzerland), PubMed ID: 32717801
7, Chen R, et al. SNAIL regulates gastric carcinogenesis through CCN3 and NEFL.Carcinogenesis, PubMed ID: 33313663
8, Sharma A, et al. Comparative Transcriptomic and Molecular Pathway Analyses of HL-CZ Human Pro-Monocytic Cells Expressing SARS-CoV-2 Spike S1, S2, NP, NSP15 and NSP16 Genes.Microorganisms, PubMed ID: 34073047
9, Sun JH, et al. Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction.Neural regeneration research, PubMed ID: 34558529
10, Wei W, et al. The Role of Ferroptosis Signature in Overall Survival and Chemotherapy of Pancreatic Adenocarcinoma.DNA and cell biology, PubMed ID: 34898275
11, Hung YH, et al. Semaphorin 4C promotes motility and immunosuppressive activity of cancer cells via CRMP3 and PD-L1.American journal of cancer research, PubMed ID: 35261797
12, Kuang Y, et al. NADH dehydrogenase subunit 1/4/5 promotes survival of acute myeloid leukemia by mediating specific oxidative phosphorylation.Molecular medicine reports, PubMed ID: 35425997
13, Burnie J, et al. P-selectin glycoprotein ligand-1 (PSGL-1/CD162) is incorporated into clinical HIV-1 isolates and can mediate virus capture and subsequent transfer to permissive cells.Retrovirology, PubMed ID: 35597982
14, Ma WL, et al. Ursolic acid silences CYP19A1/aromatase to suppress gastric cancer growth.Cancer medicine, PubMed ID: 35545835
15, Kim NY, et al. Euphorbiasteroid Abrogates EGFR and Wnt/β-Catenin Signaling in Non-Small-Cell Lung Cancer Cells to Impart Anticancer Activity.Molecules (Basel, Switzerland), PubMed ID: 35744950
16, Shonibare Z, et al. Reciprocal SOX2 regulation by SMAD1-SMAD3 is critical for anoikis resistance and metastasis in cancer.Cell reports, PubMed ID: 35905726
17, Adams LM, et al. Mapping the KRAS proteoform landscape in colorectal cancer identifies truncated KRAS4B that decreases MAPK signaling.The Journal of biological chemistry, PubMed ID: 36470426
18, Sakamoto A, et al. Transient Receptor Potential Ankyrin 1 (TRPA1) Channel Mediates Acrolein Cytotoxicity in Human Lung Cancer Cells.International journal of molecular sciences, PubMed ID: 37511605
19, Maru B, et al. PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy.Cell reports. Medicine, PubMed ID: 37683650
20, Sun Y, et al. GNA15 facilitates the malignant development of thyroid carcinoma cells via the BTK-mediated MAPK signaling pathway.Histology and histopathology, PubMed ID: 38333922
21, Wang H, et al. The regulatory role of miR-21 in ferroptosis by targeting FTH1 and the contribution of microglia-derived miR-21 in exosomes to arsenic-induced neuronal ferroptosis.Journal of hazardous materials, PubMed ID: 39186845
相关实验
负控制 negative regulation，negative control
基因的表现受到细胞内存在的特殊的调节蛋白质的阻抑，称为负调节。亦称负控制。例如某种阻抑物，当它和特异的操纵基因结合时，受该操纵基因控制的操纵子的mRNA合成就受到阻抑。这样的现象称为由阻抑物所引起的负调节。是通过调节蛋白质来调节基因作用的一种方式，与负调节相对的是正调节。
负反馈控制 negative feedback control
常规类型的负反馈是将输出信号改变符号返回送至输入端调节器，从而完成控制作用的机制。在生物的调节机制中，大多都有这种负反馈联系，从按质量作用定律进行的化学调节这样的非特异的、内在的自动控制效应开始，乃至像神经性相关、液性相关等具有特殊信息通路的控制效应都不乏其例。把输出不改变符号返回送至输入端的正反馈，在生物现象中也是意味着突然的加剧和迅速的消失，不过，这种现象并不普遍，只有在特殊场合才能看到。
contamination in real-time pcr negative control-Real-Tim
too much about it. One other thing is to check the reaction by running it on a gel, if a distinct band comes up in your negative control(and it looks like the test lanes) well that would answer your question. Good luck! Nick -methylnick- hi
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pCMV3-untagged Negative Control Vector
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