赛默飞世尔Thermo Fisher TEST TUBE R

Single tube confirmation PCR protocol

containing 60 U/ml of Zymolyase (This solution is generated by resuspending 6 mg of 100T Zymolyase in 10 mls of water. Ordering information: Seikagaku Corp., #120493-1). - Sterile yellow tips work great for this task. - Repeat this step

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

with 0.5x MBS at RT. (5)   Transfer tissues into a 1.5 ml microfuge tube using a wide-bore plastic pipette. Remove the MBS as much as possible. (6)  You may store the tissues at �80°C (use liquid nitrogen to freeze them). (7)  Add 0.5 ml of �

Single tube confirmation PCR protocol

and template. 110 µl           5 µl   10 x Taq buffer(see below) 　　11 µl         0.5 µl   20 mM dNTP's (0.2 mM)　　11 µl         0.5 µl   Taq Polymerase (2.5 units)　　638 µl   22 X   29 µl   water　　5 µl   10 µM upstream primer: A,C,or kanC (1 µM)　　5 µl   10 µM