赛默飞世尔Thermo Fisher SPOON 280MM

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

质谱样本制备技术资料

水和甲醇各涮一遍，真空干燥。 3. 胰酶储液制备：使胰酶粉末沉落瓶底，用100μ胰酶重旋液溶解，用0.5ml EP分装成5μl。 4. 50ml 100 mM NH4HCO3。 脱色酶切： 1. 切点将200μl tip头剪成吸口1.5mm左右的直径，MilliQ水涮洗一遍，吸100μl水后戳取斑点，将胶粒打入0.2ml EP管中，吸干水后再用MilliQ水洗涤两遍，吸干。 2. 脱色 180μl 50%MeOH/50mM NNH4HCO3脱色两次以上，每次半小时；180μl

A quick RNA mini-prep for Neurospora mycelial cultures

. 4. Working quickly before powder can thaw, pour or spoon ground mycelia into 15 ml round bottom Sarstedt tube (Sarstedt tubes #60.540, Princeton, NJ) containing 8 ml of E buffer at room temperature. [E buffer: 50 mM Tris-Cl pH 8.0, 300 mM NaCl, 5 mM