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999
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北京泽平
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货号:T_70122-146-652
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英文名称:STANDARD SEAL KIT ADJ 2-20UL
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文献和实验【转贴】immunofluorescence staining
do this the nail polish won't stick. Seal the edges of the coverslips with nail polish. If coverslips need to be removed for some reason, you can peel off the nail polish and "float" them off with excess TBS-T buffer.Coverslips are now stained and ready to examine
triethylamine acetate (TEAA, Cat. No. 400613) were from Applied Biosystems and the anhydrous acetonitrile (Cat. No. 40-4050-50) was from Glen Research. Trifluoroacetic acid (TFA, Cat. No. BP618-500) was from Fisher and triethylamine (TEA, Cat. No. T-0886
) was from Fisher and triethylamine (TEA, Cat. No. T-0886) was from Sigma. Fluorophores were purchased from Amersham (Cy3, Cat. No. PA23001; Cy3.5, Cat. No. PA23501; Cy5, Cat. No. PA25001) and Molecular Probes (Oregon Green 488, Cat. No. O-6147; Alexa Fluor 488
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