赛默飞世尔Thermo Fisher X100 FIOLAX

Preparation of Tonsil Lysate

a small amunt f tnsils at a time. (At this pint yu can freeze the tnsils until yu have enugh) 6.Set up the hmgenizer with the large prbe (1cm diameter). 7.Put a small amunt f minced tnsil in the 50ml Crning tube and add lysis buffer t ~10ml mark

v468 chapter 5 糖原合成酶激酶-3 的抑制

for cell lysis: 10 mM Tris-HCl, pH 7.4,50 mM NaCl, 5 mM EDTA, 50 mM NaF, 30 mM Na 4 P 2 O 7 ,and 1% (v/v) Triton-X100. 5. Protease inhibitors and phosphatase inhibitor are added fresheach time with the following final concentrations: 10 μg/mLaprotinin

Stripping Western Blots

and some other co-IPs 1% Triton X100 50mM Tris pH 7.2 10% glycerol 25mM b-glycerophosphate 2mM EDTA 2mM EGTA Triton X100 Buffer #2 - Use for high-stringency preparation of cytoskeleton (pellet) 1% Triton X100 0.27M