赛默飞世尔Thermo Fisher 缓冲液 PH2 BUF

常用缓冲液配制－2

HEPES) distilled water to 900 ml adjust pH to 7.0 distilled water to 1 liter store at 4oC 10 x Column Buffer* 500 ml 1 M PIPES Stock (250 mM PIPES) 40 ml of 0.5 M EGTA Stock (10 mM EGTA) 40 ml of 1 M MgSO4 Stock (5 mM MgSO4 )

常用缓冲液配制

Aliquote 45 ml into 50 ml PPT, store at -20 C. Oligo Buffer After preparing the following stock solutions, mix A, B, and C in a ratio of 1:2.5:1.5, repectively. Solution A Stock 2-mercaptoethanol (bME) 18 µl DXTPs (A, T, G

实验室常用缓冲液汇总

降低0.03个单位。3、10×TE Buffer （pH 7.4，7.6，8.0） □组份浓度 100 mM Tris-HCl，10 mM EDTA □配制量 1L □配置方法 1. 量取下列溶液，置于1L烧杯中。 1 M Tris-HCl Buffer（pH7.4，7.6，8.0） 100mL 500 mM EDTA（pH8.0） 20mL