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货号：T_70311769669
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文献和实验Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
of 20 mM HEPES Buffer in 0.9% saline solution. In a separate conical tube, create a T solution by adding 17 μl of the thrombin stock, and 1.3 μl of 2 N Ca solution to 135 μl of DMEM. See Table 1. 2) In a third conical tube, prepare
【转贴】immunofluorescence staining
. Store at 4C to prolong fluorescence.Reagents and Supplies:PBS & TBS-T buffers:PBS: Standard PBS for mammalian cellsTBS-T: Tris HCl 20 mM, pH 7.4 NaCl 137mM Tween-20 0.1%PEM Buffer:Stock Solution for 1 Liter:Final Concentration: 400 mM Potassium PIPES, pH
DpnI mediated site-directed Mutagenesis
uM oligo 1 0.5 µl 25uM oligo 2 0.5 µl 10mM dNTP 1.0 µl Pfu DNA polymerase (2.5 units) 1.0 µl fill w/ddH2 O to 50 µl 2. PCR conditions 95°C 30 seconds, 18 cycles of : 95°C 30 seconds, 55°C 1 minute
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