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文献和实验, A.D., Rossi, J.J. and Morris, K.V. (2005) The antisense strand of small interfering RNAs directs histone methylation and transcriptional gene silencing in human cells, RNA, 12(2): 256-262.
Chromatin Immunoprecipitation Protocol for Histone Modification
, as well as with U2OS, 293, Hela, T98G cells from human. Nonetheless, we would urge each investigator to expend much effort in standardizing the method for their specific experimental system. Back to top Procedure Typically, when making a lysate
silencing (TGS) in Human cells (Matzke and Birchler 2005; Morris 2005) has illustrated the fact that RNA may be far more intricately involved in epigenetics than was previously assumed. To determine more clearly how siRNAs are interacting with the homologous
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