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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production
Harvest more feeder cells for your next expansion. Put 0.5 mL f feeder cells in DMEM/20 FCS (note the removal of the HT) in a T25 flask (one for each positive clone) for later use When the cells are ready for expansion out of the 24-well plate
In the field of therapeutic recombinant proteins, monoclonal antibodies (mAbs) have achieved a rising success with more than 30 mAbs that have reached the market in the past 20 years. From a structural standpoint, one of the most important
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