13C20,15N10-Cyclic di-GMP (sod

Crystallization of Kinesin Family Motor Proteins

diffusion2 microliters of protein (15 mg/ml) + 2 microliters of reservoir buffer (RB1) composed of 30% w/v PEG4000, 100 mM Tris-HCl pH 8.5, 200 mM sodium acetate, 8% w/v sucrose, 4 mM ADP, 10 mM MgCl2, equilibrated against RB1 for 5 dKikkawa et al., 2001KIF

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

  Figure 14.9.6 Click synthesis of the biotinylated c‐di‐GMP conjugate 22 from the biotinylated azide 11 and mono‐6‐thioated analog of 2′‐ O ‐propargylated cyclic‐di‐GMP (21 ). Abbreviation: Gua, guanin‐9‐yl

In Vitro Transcription Assay (Run-off Assay) using Permeabil

of absolute Ethanol). 15. Incubate for 1 hr at -20℃ to precipitate the RNA. 16. Centrifuge at maximum speed (13,000 rpm) in a microcentrifuge for 10 min at 4℃ to pellet the RNA. Remove the supernatant. 17. Add 1 ml of 75% Ethanol to wash the RNA pellet. 18