Gonadoliberin 3 x 10 ug

交联染色质免疫共沉淀（X-ChIP）实验设计

冻存于-20°C.样品采用蛋白酶K处理可使相邻肽段剪切成羧基团的芳香族氨基酸和脂肪族氨基酸。蛋白质和DNA之间的交联通过DNA的纯化被打断。3检测DNA浓度，取5ul纯化的DNA到995ulTE中得到一个原溶液的200倍稀释测其OD260。那么DNA的浓度就为OD260x10,000.μg/ml。这样就可计算出染色质制品的DNA浓度。4 免疫共沉淀1 从2.2得来的染色质制品，推荐每个IP样品都采用25ug的DNA的量。每个样品按1：10的量加入RIPA溶液。需要一个样本作为空珠子的对照。2在除空

Staining Methods for cell death Z. Xia 10/2/95

gently tilt the plates to mix R.T. 15 min Aspirate off media Staining: 4 ug/ul P. I./0.1 % triton X-100/0.5 mg/ml RNaseA in PBS R.T.5 min Examine under the scope or mount with coverslips Note: P

RNA Purification Protocol for 10-20mg of Animal Tissue

# SSI-1015-39). 3. Add 200 μl of PNP Buffer to the cell lysate. Mix the sample gently by inverting the tube 10 times. 4. Place the tube on ice for 5 minutes. 5. Centrifuge at max speed (.13,000 x g) for 3 minutes at room temperature