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文献和实验Protein Carbonyl Determination Using Biotin Hydrazide
Protein oxidation is a recognized component of aging and a consequence of severe or prolonged exposure to reactive oxygen species (ROS). Direct attack of protein by ROS causes formation of protein-bound carbonyl groups (1 ). These carbonyl
RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
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