Sulfhydryl-Reactive Resin 15 m

Immunofluorescence on ultrathin resin sections

. Residual water is drained with filter paper along the outside of the loop. Note: air-dried resin sections can be stored for months prior to labelling. Procedure: Encircle sections

Coupling Peptides To Resin For Affinity Purification

volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add

Mag-Bind Blood DNA Maximum Yield Protocol (2ml-6ml)

. Add 10ml SPM Buffer to the sample. 14. Resuspend the Mag-Bind particles pellet by vortexing. Incubate 3 minutes at room temperature. Complete resuspension of the Mag- Bind particles pellet is critical to obtain good results. 15. Place