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- LC6203-10amps
- 2025年07月13日
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10 x 1.0 mL
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文献和实验A Proteomics Approach to Identify Redox-Sensitive Proteins
-Rad). 10. CB-X Protein Assay kit (G-Biosciences). 11. Reduction buffer: 50 mM Tris–HCl, pH 8.0, 7 M urea, 2 M thiourea, 1% CHAPS, and 1 mM DTT. 12. 11 cm ReadyStrip IPG strips (pH 4–7) (Bio-Rad). 13. SDS-PAGE running buffer (10×): 250
【转贴】immunofluorescence staining
6.8 200 mL. 80 mM 0.5 M EGTA, pH 7.0 10 mL. 5 mM 1.0 M MgCl2 2 mL 2 mMFilter sterilize and store at 4 C.Formaldehyde: Use EM grade formaldehyde - it can be obtained from the following suppliers:Polysciences, Cat. #18814, 10 mL. ampules, 16%EM Sciences, Cat
反向微柱的准备Preparation of Reversed-Phase Microcolumns
with peptides generated from poorly abundant, gel-separated proteins. Apply 20 µL of 0.1% TFA to the top of the column. Equilibrate the column by gently pushing 10 µL of 0.1% TFA through it, using gentle air pressure generated
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