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- 2025年07月10日
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- 文献和实验
- 技术资料
- 库存：
999
- 供应商：
北京泽平
- 现货状态：
热销品大量现货，其余请咨询
- 保修期：
1年
- 规格：
EA
货号：T_70312513327
中文名称：
英文名称：X10 WASH BOTTLES GRADUATED, 500 M
货期：热销品现货，其他请咨询
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文献和实验Clone Genes From a Phage Library
nylon filters from Fisher (MSI N00HY08250, 82 mm, 50/box). These are quite cheap. Solutions: LB, SM solution, Church hybridization solution, Church wash solution, Chloroform (see the end for recipes).Ex-assist phage as well for plasmid excision
Large Scale Tubulin Preparation
onto PC column 15. Collect flowthrough from PC column, aliquot and freeze at -800 C II. Buffers & Nucleotides PB (Pipes/Polymerization Buffer) : 0.1 M K-Pipes, pH 6.8, 0.5 mM MgCl2 , 2 mM EGTA, 0.1 mM EDTA, 0.1 % b
Large Scale Tubulin Preparation
2. Collect homogenate (~8-9 liters), transfer into 36 GSA bottles and spin 90' at 12K in a GSA rotor at 4¡C. 3. Collect supernatant and transfer 1 liter to a 1.8L glass Fernbach flask that has 500 ml of 37¡C glycerol. Add 0.1 mM GTP, 0.5 mM ATP
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