GPR56 Extracellular domain Ant

Construction of a Human Antibody Domain (VH) Library

), called a “domain antibody”, or “dAb”, is approximately four times smaller than a Fab and half the size of a scFv. Here, we describe the construction of a phage-displayed VH library and an approach to introduce genetic diversity in this library, where both diverse

Expression of Extracellular N-Terminal Domain of NMDA Receptor in Mammalian Cells

consists of two functionally distinct regions: a large amino-terminal extracellular domain containing the putative agonist binding site and a hydrophobic core consisting of four transmembrane-spanning domains that participate in the formation of the ion

Improvement of Single Domain Antibody Stability by Disulfide Bond Introduction

antibody repertoire. By introducing this particular disulfide bond within a single domain antibody, the conformational stability can be increased in general. In this chapter it is described how to introduce this extra intra-domain disulfide bond