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文献和实验[分享] Strip Antibodies and Re-use Protein blots membrane
Western blotting is a commonly used technique to study protein structure and function. Typically, protein samples are electrophoresed on SDS-gels and transferred to solid support (nitrocellulose or PVDF membranes) for subsequent probing
Membrane Transfer and Crosslinking for RNA
). Northern assays require the total RNA to be resolved on a denaturing agarose gel first, then transferred to a membrane and immobilized for subsequent hybridization. Nonisotopic RPAs, which utilize probes labeled with modified nucleotides (e.g. biotin
Single Quantum Dot Tracking of Membrane Receptors
fluorophores and fluorescent proteins rapidly photobleach, whereas gold particles and latex beads, although more stable, are bulky and usually stain only one protein per experiment. In comparison, QDs are bright and photostable fluorescent probes with a size
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