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文献和实验Comparison of Competitive PCR and Positive Control-Based PCR
The exponential amplification of small amounts of nucleic acids makes polymerase chain reaction (PCR) not only powerful but also challenging as a quantitative method. Variations in nucleic acid preparation, thermal cyclic performance
Major progress has been made in unravelling of regulatory mechanisms in eukaryotic cells. Modification of target protein properties by reversible phosphorylation events has been found to be one of the most prominent cellular control processes
whereas typical macrophages are strongly positive all over the cytoplasm. When looking at forward/side scatter profiles in the fluorescence-activated cell sorter (FACS), DCs show high light scattering and are outside the typical lymphocyte gate. Functionally
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