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文献和实验Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
Autoradiography of Peptide Receptors
, the most widely used of which is in vitro receptor autoradiography. The methods are relatively simple and versatile and are applicable to the analysis of secondary messenger systems, ion channels, and enzymes (see Chapter 11 ) as well as receptors and may be used
chromatography with electrochemical detection or with salicylate treatment (5 –11 ), and immunohistochemistry (12 –17 ). Recently, a nucleic acid probe has been generated that binds to an abnormal guanine base, 8-hydroxyguanine (oh8G) (Fig. 1 ) (18
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