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文献和实验Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
a method to evaluate the effect that small molecules have on the binding interactions of a nuclear receptor protein with a series of peptides. The multiplexed microsphere?based system employs peptide?coupled microsphere populations that are fluorescently
Blocking of unwanted non-specific staining
by acting on primary Abs. 5- Wash once in TBS-T (residual milk can contribute to blocking). Blocking of endogenous Fc blocking. Specimens not paraffin embeddded may have significant Fc binding activity by macrophages, B cells, T cells
Characterization of 5‐HT1A,B and 5‐HT2A,C Serotonin Receptor Binding
Kenakin, T. 1997. Pharmacologic analysis of drug‐receptor interaction. 3rd edition. Raven Press, New York. Kennett, G.A., Wood, M.D., Bright, F., Trail, B., Riley, G
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