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文献和实验Imaging B-Cell Receptor Signaling by Single-Molecule Techniques
B-cell activation initiates antibody responses against pathogens. Recent imaging of B cells in vivo shows that B cells move rapidly through lymphoid tissues to search for antigens captured on the surfaces of antigen-presenting cells
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow
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