赛默飞世尔Thermo Fisher COMB 20 SAM

SEQUENCING GELS

the plates with a metal spatula by prying from one bottom corner.  6% gels can be lifted from the glass with a piece of 3MM paper rubbed over the gel - then covered with saran wrap & dried on a gel dryer.  8-20% gels are lifted using a used piece of X-ray

SDS-PAGE

for routine analytical gels, and 1.5 mm spacers to run more sample. I usually use a comb that makes 20 wells.2. Solutions:I. 1.5 M Tris-HCl, pH 8.8II. 1 M Tris-HCl, pH 6.8III. 30% acrylamide/bisacrylamide (37.5:1). This solution can be bought commercially

DNA Sequencing

and the samples stored at -20℃ for later use. DNA sequencing gels Gels used for DNA sequence analysis are of the wedge type. These produce a voltage gradient which decreases as DNA migrates down the gel, thus retarding the rate of migration of smaller fragments