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文献和实验Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
Purification Of Anti Peptide Antibody
peptide to the resin. Thaw serum- dilute 1:1 with TBS and filter through a 0.2 um filter Load the serum over the column, taking at least 20 minute total. Run the breakthrough over the column five times. Alternatively you can use
Mutational Analysis of the Human Protein C Gene
(2 ,3 ). The gene is approximately 11 kb long and is composed of 9 exons and 8 introns. In common with factors VII, IX and X the exons encode specific structural domains of the protein C molecule. Exon 1 encodes the 5′ untranslated region, exon 2 encodes the signal
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