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文献和实验secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
Staining for Surface Receptors
up" on the coverslip, and the wet whatman paper helps prevent the antibody from drying up. 9. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse
)1:1000. 9. Wash 3x. 10. Second Blotto block as above, 5'. 11. Aspirate Blotto, and apply secondary Ab. Incubate 20' in the dark. Dilute antibody with Blotto. Cy3 donkey anti-mouse (Jackson Immunoresearch ) 1:500, FITC goat anti-mouse 1:500
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