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- Fabgennix
- PC-PLA2G2A
- 2025年07月04日
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文献和实验Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2
Phospholipases A2 (PLA2 ) are a family of ubiquitous lipolytic enzymes that are found both as intracellular and secreted proteins. Secretory PLA2 s are small (14 kDa), homologous proteins that require millimolar Ca2+ for catalytic activity
Phospholipase A2 Purification and Characterization: A Case Study
2 from chicken pancreatic (ChPLA2-IB) and chicken intestinal (ChPLA2-IIA) origins. Interestingly, ChPLA2-IB hydrolyzes efficiently both purified PC and PE, whereas ChPLA2-IIA hydrolyzes only PE and not PC, even after a long incubation period. These analytical
BioBrick assembly over standard assembly are that it is faster, does not require restriction digestions or ligations or DNA extraction from a gel, and is more flexible in the sense that there is more control over the exact engineered sequence
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