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文献和实验Rachel1123 各位大侠,我的实验需要用一种抑制剂抑制Stat3的表达,查了merck公司的Stat3 inhibitors产品,种类很多,不知该选用哪一种? chemicals 跟inhibit peptide 有什么区别? 谢谢!:) ylhuang0502 对于Stat3我是外行,但是我用过很多种其他的抑制剂,选择的方法和思路应该是一致的。 1.抑制剂一般是首先要考虑特异性,自然是越高
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
importance to employ a control to determine that the staining pattern is specific. One of the frequently used controls in IHC is a so-called absorption control: phospho-specific primary antibodies are first incubated with a phospho-peptide immunogen to block
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
the phosphorylation site; therefore, substrate?directed, phosphorylation?state?sensitive, motif?specific (?phospho?motif?) antibodies represent powerful tools to identify novel kinase substrates and to investigate mechanisms of substrate phosphorylation
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