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文献和实验Comparison of Competitive PCR and Positive Control-Based PCR
The exponential amplification of small amounts of nucleic acids makes polymerase chain reaction (PCR) not only powerful but also challenging as a quantitative method. Variations in nucleic acid preparation, thermal cyclic performance
Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads
importance to employ a control to determine that the staining pattern is specific. One of the frequently used controls in IHC is a so-called absorption control: phospho-specific primary antibodies are first incubated with a phospho-peptide immunogen to block
Purification and Identification of Protein Complexes That Control the Cell Cycle
The human Rad9-Hus1-Rad1 (9-1-1) and Rad17-RFC40-RFC38-RFC37-RFC36 (hRad17-RFC) protein complexes are important components of the mammalian deoxyribonucleic acid (DNA) damage response. Accumulating evidence from biochemical and molecular
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