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文献和实验. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0 b) Determine and record A280 and A492. c) Calculate
Single Primer ("Semi-Random") P
cresol red 2. T/TS 10.5 µL EDB 1 µL Taq Polymerase (Promega) @ 5 u/µL 1 µL TaqStart antibody (CloneTech) as delivered 3. EDB 2.5 mg/ml bovine serum albumin in 10 mM Tris, pH 8.3 References 1. S.R.J.A.M. Hermann
, you can determine the FITC to Protein ratio. When Biotinylating an antibody, you can test the antibody by Flow Cytometry. 6. F/P Ratio: a) Dilute an aliquot of FITC-Ig complex with buffer so that the Absorbance at 280nm (A280) is less than 2.0
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