BTB/POZ domain-containing prot

Protein A Purification of Antibody

fractions until base line is achieved.Elute Ab with 5 bed volumes of Elution Buffer. Collect 1 ml fractions into 50 ml 1M Tris, until base line is achieved.Take 50 ml aliquots of each fraction for ELISA.Pool fractions containing Ab. Dialyze against 2 x 4L

Intracellular Antibody Capture (IAC) Methods for Single Domain Antibodies

. We describe here the new generation protocol for intracellular antibody capture to facilitate selection of functional single domains. This protocol uses a series of optimized single domain libraries, based on designed intracellular variable (VH or VL) region scaffolds

Single-Domain VH Antibody Fragments from a Phage Display Library

The interaction between antigens and antibodies can be viewed as a protein engineering system that was optimized by nature and is capable of recognizing any given molecular entity (antigen). Antigen recognition is conferred to the antibody