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文献和实验We have been karyotyping animals in the Grainger lab to confirm that the stocks maintained in the lab are indeed X. tropicalis and to establish that wild-caught animals being used for gynogenesis are also X. tropicalis . We recently
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
(9) Sonicate the homogenate on ice (10 sec. pulse + 50 sec. break x 6 times, 20% amplitude with a small tip of Branson sonifier (Fisher Dismembranator Model 500)). This step releases chromatin DNA from cells without solubilizing yolk proteins that make
Application of Multi-PRINS to Simultaneously Identify Chromosomes 18, X, and Y in Prenatal Diagnosis
to chromosomes 18, X, and Y were shown as yellow, red, and green color spots, respectively. Multi-PRINS is as accurate and reliable as multicolor fluorescent in situ hybridization (multi-FISH) for the detection of aneuploidies involving chromosomes 18, X, and Y
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