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文献和实验Identification of Deacetylase Substrates with the Biotin Switch Approach
techniques are continuously improving, they can be limited by the narrow and many times unknown epitope specificity of Kac antibodies. An alternative approach is the biotin switch capture of deacetylated proteins. Similar in part to other biotin switch
Studies of the Ubiquitin Proteasome System
phosphatase inhibitors and 50 µM MG132 (or lactacystin) Anti‐E3 or anti‐substrate antibody Nondenaturing Triton X‐100 wash buffer (see recipe ) Tris‐buffered saline (TBS
酶活化,DNA降解,细胞DNA减少,因而可在G0/G1峰前出现一个亚二倍体峰,也就是凋亡峰。 lard:除左跑gel 之外最平就是他了. (PI 一個flow sample 才RMB 0.6, 不計机的錢)。其實跑gel+PI 已夠說明那東西是會引起apoptosis。用antibody/kit 都是另有要求. shaman3:其实,PI分不清晚期凋亡和已经死的细胞,在做有关凋亡的流式检测的时候利用的是磷脂酰丝氨酸外翻分析(Annexin V法) 磷脂酰丝氨酸(Phosphatidylserine
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