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文献和实验Site‐Specific Protein Labeling with SNAP‐Tags
BG‐PEG‐Biotin Probes Support Protocol 2: Synthesis of BG‐800 Substrate Basic Protocol 3: In Vitro Labeling of SNAP‐Tag Fusions Support Protocol 3: Labeling of SNAP‐Tag Proteins
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
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