赛默飞世尔Thermo Fisher X50 Syringe

Construction and Characterization of Adenovirus Vectors

stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

Procedures for In Vitro Production of Bovine Embryos

bicarbonate and 45 ml Na lactate (Stock 1 ). 2. Stir until bicarbonate dissolves. 3. Add 18 ml Percoll . 4. Add 79 ml MgCl2 (Stock 12 ) and 39 ml CaCl2 (Stock 13 ). 5. While stirring, adjust pH to 7.3-7.45 and filter with a 0.45 mm filter (50