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文献和实验Characterizing Bile Acid and Lipid Metabolism in the Liver and Gastrointestinal Tract of Mice
., Downes, M., Yu, R.T., Shelton, J.M., Richardson, J.A., Repa, J.J., Mangelsdorf, D.J., and Kliewer, S.A. 2006. Regulation of antibacterial defense in the small intestine by the nuclear bile acid receptor. Proc. Natl. Acad. Sci. U.S.A. 103:3920‐3925
secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
Dual- and Triple-Color Fluorospot
in several publications and reagents/kits for various cytokine combinations and species are commercially available. The most common protocol for fluorospot includes the use of biotinylated detection antibodies for one cytokine and an FITC-labeled detection antibody
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