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文献和实验Two dimensional peptide mapping
This specfic protocol is the latest incarnation of peptide mapping procedures that have been developed here in the TVL/MBVL of the Salk Institute over the last 20 years. Wade Gibson developed the first peptide mapping protocol
Synthesis of C-Terminal Peptide Thioesters Using Fmoc-Based Solid-Phase Peptide Chemistry
This chapter provides two protocols for the solid-phase synthesis of peptide thioesters using N α -Fmoc-protected amino acids. The first protocol is based on a so-called safety-catch linker, while the second relies on a backbone amide
Blocking of unwanted non-specific staining
receptors). Crossreactivity of the secondary reagents with endogenous proteins. Blocking of endogenous enzymes Endogenous enzymes such as AlkPhos, AcPhos and esterases are destroyed by boiling Ref, even a short time at 100 C. Peroxidase
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