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文献和实验Coupling of Monoclonal Antibodies with Biotin
glycoprotein of 66,000–68,000 mol wt, found in egg albumin and in avian tissues. The interaction between avidin and biotin occurs rapidly, and the stability of the complex has prompted its use for in situ attachment of labels in a broad variety
Cell Surface Engineering of Mesenchymal Stem Cells
a stepwise process including (1) treatment of cells with sulfonated biotinyl-N -hydroxy-succinimide to introduce biotin groups on the cell surface, (2) addition of streptavidin that binds to the biotin on the cell surface and presents unoccupied binding sites
High-Affinity Immobilization of Proteins Using Biotin- and GST-Based Coupling Strategies
focuses on high-affinity immobilization of protein substrates for affinity and kinetic analyses using biotin/streptavidin interaction and GST/anti-GST-antibody interaction.
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