ACE2 Protein, Human (Biotinyla

Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag

function. Many R proteins are associated with the plasma membrane (PM) and expressed at low levels. Here, we describe a method to purify such low-abundance PM R protein �complexes from Arabidopsis using a biotinylated affinity tag, called the HPB tag

Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of Their Protein Function

1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable

Western Blotting with Biotinylated Antibodies

SAMPLE PREPARATION For Protein Concentration Determination of Cell Culture 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS). 2.Aspirate excess PBS. 3.Add 1ml boiling lysis buffer