- ￥2450 - 5400
- MedChemExpress(MCE)
- 中国
- HY-P78189-20μg
- 2025年07月15日
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- 供应商：
广州威佳科技有限公司
- 规格：
20μg/50μg
| 规格： | 20μg | 产品价格： | ￥2450.0 |
|---|---|---|---|
| 规格： | 50μg | 产品价格： | ￥5400.0 |
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文献和实验Purification of Resistance Protein Complexes Using a Biotinylated Affinity (HPB) Tag
function. Many R proteins are associated with the plasma membrane (PM) and expressed at low levels. Here, we describe a method to purify such low-abundance PM R protein �complexes from Arabidopsis using a biotinylated affinity tag, called the HPB tag
1 in the human embryonic kidney (HEK293) cell line are introduced. In isolated cell culture mitochondria, the proton leak can be measured and modulators of UCP1 activity can be tested. As mouse UCP1 in this system shows native behaviour, this may be a suitable
Western Blotting with Biotinylated Antibodies
. 3.Centrifuge the homogenate (16,000 x g, 15 C) for 5 minutes to pellet insoluble material, then discard pellet. 4.Dilute an aliquot of the tissue lysate sample at least 10-fold for the BCA (Pierce) protein concentration assay. POLYACRYLAMIDE GEL
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