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- MedChemExpress(MCE)
- 中国
- HY-P0327A-1mg
- 2025年07月13日
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文献和实验Calmodulin-Binding Peptide as a Removable Affinity Tag for Protein Purification
Protein purification is an important tool for investigations on protein function, structure analysis, and biotechnological use. Therefore a number of different techniques have been developed for fast, reliable, and reproducible overexpression
T7 Lytic Phage-Displayed Peptide Libraries: Construction and Diversity Characterization
In this chapter, we describe the construction of T7 bacteriophage (phage)-displayed peptide libraries and the diversity analyses of random amino acid sequences obtained from the libraries. We used commercially available reagents, Novagen’s T7
/L of functional protein. Herein, we report on the production in Escherichia coli and the purification of a recombinant mature streptavidin bearing a T7-tag. Optimization of critical parameters, including the glucose concentration, the pH and the time
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