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文献和实验Signalosome Profiling Reveals Allosteric Interactions Between G Protein-Coupled Receptors
do not function in living cells as monomers, but most likely dimers, or even larger arrays of receptors. Standard drug design approaches rely on the notion that drugs binding the two receptors in a given dimer likely function independently
prior to rehydration with enzyme.After digestion,the peptides are extracted from the gel and mass analyzed.In-gel digestion procedureMaterials25 mM NH4HCO3 in 50% acetonitrile25 mM NH4HCO3,pH 85% TFA/ 50% acetonitrile0.1 mg/ml trypsin (sequence grade,Promega)in 25 mM
.These are only rough guidelines. Detailed instructions about PCR laboratory setup and maintenance may be found in PCR Methods and Applications, 3, 2, S1-S14, 1993.Preparation of Reaction Mixture To perform several parallel reactions, we recommend the preparation
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